In vivo nuclear delivery of oligonucleotides via hybridizing bifunctional peptides
نویسندگان
چکیده
منابع مشابه
Drug delivery of oligonucleotides by peptides.
Oligonucleotides are promising tools for in vitro studies where specific downregulation of proteins is required. In addition, antisense oligonucleotides have been studied in vivo and have entered clinical trials as new chemical entities with various therapeutic targets such as antiviral drugs or for tumour treatments. The formulation of these substances were widely studied in the past. With thi...
متن کاملIn vivo delivery of transcription factors with multifunctional oligonucleotides
Therapeutics based on transcription factors have the potential to revolutionize medicine but have had limited clinical success as a consequence of delivery problems. The delivery of transcription factors is challenging because it requires the development of a delivery vehicle that can complex transcription factors, target cells and stimulate endosomal disruption, with minimal toxicity. Here, we...
متن کاملIn vivo delivery of phosphorothioate oligonucleotides into murine retina.
OBJECTIVES To determine the fate of phosphorothioate oligonucleotides (S-ODNs), which are commonly used for antisense strategy, in murine retina in vivo with the use of fluorescein isothiocyanate (FITC)-labeled S-ODNs, and to evaluate our fusogenic liposome system that may facilitate the delivery of S-ODNs. METHODS The FITC-labeled S-ODNs were encapsulated in liposomes, which were then coated...
متن کاملRedirecting splicing with bifunctional oligonucleotides
Ectopic modulators of alternative splicing are important tools to study the function of splice variants and for correcting mis-splicing events that cause human diseases. Such modulators can be bifunctional oligonucleotides made of an antisense portion that determines target specificity, and a non-hybridizing tail that recruits proteins or RNA/protein complexes that affect splice site selection ...
متن کاملIntracellular Delivery of Proteins via Fusion Peptides in Intact Plants
In current plant biotechnology, the introduction of exogenous DNA encoding desired traits is the most common approach used to modify plants. However, general plant transformation methods can cause random integration of exogenous DNA into the plant genome. To avoid these events, alternative methods, such as a direct protein delivery system, are needed to modify the plant. Although there have bee...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Gene Therapy
سال: 2001
ISSN: 0969-7128,1476-5462
DOI: 10.1038/sj.gt.3301345